Kuan Hu,Hao Geng,Qingzhou Zhang,Qisong Liu,Mingsheng Xie,Chengjie Sun,Wenjun Li,Huacan Lin,Dr. Fan Jiang,Prof. Tao Wang,Prof. Yun-Dong Wu,Prof. Zigang Li
First published: 11 May 2016Full publication history
DOI: 10.1002/anie.201602806View/save citation
Cited by: 0 articles last updated 11 May 2016
Abstract
The addition of a precisely positioned chiral center in the tether of a constrained peptide is reported, yielding two separable peptide diastereomers with significantly different helicity, as supported by circular dichroism (CD) and NMR spectroscopy. Single crystal X-ray diffraction analysis suggests that the absolute configuration of the in-tether chiral center in helical form is R, which is in agreement with theoretical simulations. The relationship between the secondary structure of the short peptides and their biochemical/biophysical properties remains elusive, largely because of the lack of proper controls. The present strategy provides the only method for investigating the influence of solely conformational differences upon the biochemical/biophysical properties of peptides. The significant differences in permeability and target binding affinity between the peptide diastereomers demonstrate the importance of helical conformation.
Funded by
National Natural Science Foundation of China. Grant Numbers: 21102007, 31300600, 21372023
MOST. Grant Numbers: 2015DFA31590, 2013CB911500
Shenzhen Science and Technology Innovation Committee. Grant Numbers: SW201110018, SGLH20120928095602764, ZDSY20130331145112855, KQCX20130627, JSGG20140519105550503, KQCX20130627103353535
Shenzhen Peacock Program. Grant Number: KQTD201103
